Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA.
Tissue Eng Part C Methods. 2011 Dec;17(12):1223-32. doi: 10.1089/ten.tec.2011.0134. Epub 2011 Sep 6.
Platelets are specialized cells produced by megakaryocytes in the bone marrow that represent the first defense against hemorrhage, yet they also play a pathological role in thrombosis, inflammation, and cancer. Millions of platelet transfusions are conducted each year, and the supply of this blood component is limited. There are many diseases where platelet production or function is impaired with severe consequences for patients. With such clinical need, new insight into the formation of platelets would have a major impact on patients and healthcare. We developed an innovative 3D system to study platelet production that represents the first spatial reconstruction of the bone marrow environment. In this system human megakaryocytes were able to migrate toward the vascular niche, extend proplatelets, and release functional platelets into vascular tubes. The combination of different bone marrow components and the compliance of silk-based vascular tubes makes this model a unique tool for the study of platelet formation and production for use in healthcare needs.
血小板是骨髓中巨核细胞产生的专门细胞，是防止出血的第一道防线，但它们在血栓形成、炎症和癌症中也起着病理性作用。每年都要进行数百万次血小板输注，而这种血液成分的供应是有限的。许多疾病都会导致血小板的产生或功能受损，给患者带来严重后果。鉴于这种临床需求，对血小板形成的新认识将对患者和医疗保健产生重大影响。我们开发了一种创新的 3D 系统来研究血小板的产生，这代表了对骨髓环境的首次空间重建。在这个系统中，人类巨核细胞能够向血管龛迁移，延伸出血小板前体，并将功能性血小板释放到血管管腔中。不同骨髓成分的组合和基于丝的血管管腔的顺应性使该模型成为研究血小板形成和产生的独特工具，可用于医疗保健需求。
