Department of Chemistry, Center of Natural and Exact Sciences, Federal University of Santa Maria, Santa Maria-RS, Brazil.
Nicotine Tob Res. 2011 Dec;13(12):1210-9. doi: 10.1093/ntr/ntr154. Epub 2011 Sep 6.
The biological systems of both smoker and passive smoking suffer changes caused by toxic compounds from cigarette smoke such as inflammation, lipid peroxidation, and deficiency of vitamin E. The aim of the present study was to evaluate the effect of vitamin E on acetylcholinesterase (AChE) activity and the lipid peroxidation level in the brain of rats in the model of exposure to aged and diluted sidestream smoke (ADSS).
Adult male Wistar rats (200-300 g) were exposed to ADSS for 4 weeks and treated with vitamin E (50 mg/kg/day) loaded by gavage. In the first, second, third, and fourth weeks, animals were concomitantly exposed to the smoke of 1, 2, 3, and 4 cigarettes/day, respectively. The duration of each exposure was 15 min, daily.
For rats exposed to ADSS, the AChE activity and lipid peroxidation level increased in the striatum, cerebral cortex, and cerebellum. In contrast, the activity of AChE and the level of lipid peroxidation decreased in the smoke group treated with vitamin E.
The results suggest that the rats exposed to ADSS and treated with vitamin E significantly reduced the raised activity of AChE and level lipid peroxidation from the brain structures studied. The study, therefore, concludes that vitamin E could be considered as a therapeutic agent in this type of exposure.
吸烟和被动吸烟都会导致生物系统发生变化，香烟烟雾中的有毒化合物会引起炎症、脂质过氧化和维生素 E 缺乏等变化。本研究旨在评估维生素 E 对暴露于陈旧和稀释侧流烟雾（ADSS）模型大鼠大脑中乙酰胆碱酯酶（AChE）活性和脂质过氧化水平的影响。
成年雄性 Wistar 大鼠（200-300 g）接受 ADSS 暴露 4 周，并通过灌胃给予维生素 E（50 mg/kg/天）治疗。在第一、第二、第三和第四周，动物分别同时暴露于 1、2、3 和 4 支香烟/天的烟雾中，每次暴露 15 分钟，每天一次。
对于暴露于 ADSS 的大鼠，纹状体、大脑皮层和小脑的 AChE 活性和脂质过氧化水平增加。相比之下，用维生素 E 治疗的烟雾组中 AChE 的活性和脂质过氧化水平降低。
研究结果表明，暴露于 ADSS 并用维生素 E 治疗的大鼠可显著降低所研究脑结构中升高的 AChE 活性和脂质过氧化水平。因此，研究得出结论，维生素 E 可被视为这种暴露类型的治疗药物。
