Zang Yanqing, Sato Hideyo, Igarashi Kiharu
Course of Science of Bioresources, The United Graduate School of Agricultural Science, Iwate University.
Biosci Biotechnol Biochem. 2011;75(9):1677-84. doi: 10.1271/bbb.110168. Epub 2011 Sep 7.
The anti-diabetic effects of a kaempferol glycoside-rich fraction (KG) prepared from leaves of unripe Jindai soybean (Edamame) and kaempferol, an aglycone of kaempferol glycoside, were determined in genetically type 2 diabetic KK-A(y) mice. The hemoglobin A(₁c) level was decreased and tended to be decreased by respectively feeding KG and kaempferol (K). The area under the curve (AUC) in the oral glucose tolerance test (OGTT) tended to be decreased by feeding K and KG. The liver triglyceride level and fatty acid synthase activity were both decreased in the mice fed with KG and K when compared to those parameters in the control mice. These results suggest that KG and K would be useful to improve the diabetes condition. The major flavonoids in KG were identified as kaempferol 3-O-β-D-glucopyranosyl(1→2)-O-[α-L-rhamnopyranosyl(1→6)]-β-D-galactopyranoside, kaempferol 3-O-β-D-glucopyranosyl(1→2)-O-[α-L-rhamnopyranosyl(1→6)]-β-D-glucopyranoside, kaempferol 3-O-β-D-(2-O-β-D-glucopyranosyl) galactopyranoside and kaempferol 3-O-β-D-(2,6-di-O-α-L-rhamnopyranosyl) galactopyronoside, suggesting that these compounds or some of them may be concerned with mitigation of diabetes.
从未成熟的仁井田大豆（毛豆）叶子中制备的富含山奈酚糖苷的组分（KG）以及山奈酚糖苷的苷元山奈酚对2型糖尿病遗传模型KK-A(y)小鼠的抗糖尿病作用进行了测定。分别喂食KG和山奈酚（K）后，血红蛋白A₁c水平降低且有降低趋势。口服葡萄糖耐量试验（OGTT）中的曲线下面积（AUC）在喂食K和KG后有降低趋势。与对照小鼠的这些参数相比，喂食KG和K的小鼠肝脏甘油三酯水平和脂肪酸合酶活性均降低。这些结果表明，KG和K可能有助于改善糖尿病病情。KG中的主要黄酮类化合物被鉴定为山奈酚3-O-β-D-吡喃葡萄糖基(1→2)-O-[α-L-鼠李吡喃糖基(1→6)]-β-D-吡喃半乳糖苷、山奈酚3-O-β-D-吡喃葡萄糖基(1→2)-O-[α-L-鼠李吡喃糖基(1→6)]-β-D-吡喃葡萄糖苷、山奈酚3-O-β-D-(2-O-β-D-吡喃葡萄糖基)吡喃半乳糖苷和山奈酚3-O-β-D-(2,6-二-O-α-L-鼠李吡喃糖基)吡喃半乳糖苷，表明这些化合物或其中一些可能与糖尿病的缓解有关。
