Yang Miyoung, Song Myoung-Sub, Kim Sung-Ho, Kim Jong-Choon, Kim Joong-Sun, Shin Taekyun, Moon Changjong
Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National, University, Gwangju 500-757, Korea.
J Vet Sci. 2011 Sep;12(3):203-7. doi: 10.4142/jvs.2011.12.3.203.
This in vitro study evaluated the detrimental effect of acute gamma (γ)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy γ-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after γ-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose-dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances significantly blocked γ-irradiation-induced cytotoxicity in immature hippocampal neurons. The results suggest that the caspase-dependent cytotoxicity of γ-rays in immature hippocampal cultured neurons may be caused by oxidative stress.
这项体外研究评估了急性γ射线照射对大鼠未成熟海马神经元的有害影响。将大鼠未成熟海马神经元（体外培养0.5天）用0~4 Gy的γ射线进行照射。在γ射线照射后24小时，使用乳酸脱氢酶释放试验分析细胞毒性。未成熟海马神经元中辐射诱导的细胞毒性呈剂量依赖性增加。促凋亡半胱天冬酶抑制剂和抗氧化物质的预处理显著阻断了γ射线照射诱导的未成熟海马神经元的细胞毒性。结果表明，未成熟海马培养神经元中γ射线依赖半胱天冬酶的细胞毒性可能是由氧化应激引起的。
