Singh N K, Ghosh S
Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, Bareilly, UP, India.
Exp Appl Acarol. 2003;31(3-4):297-314. doi: 10.1023/b:appa.0000010382.73735.23.
In India, control of tick infestations is a major problem facing the owners of highly productive crossbred cattle. To develop suitable immunoprotective measures against the multitick infestations, specific problem encountered by the Indian farmers, an attempt has been made to identify candidate protein molecules in the larvae of Hyalomma anatolicum anatolicum and Boophilus microplus. Employing two steps affinity chromatography method, glycoproteins of 34 and 29 kDa were isolated from H. a. anatolicum and B. microplus, respectively. The additive effect of the isolated glycoproteins was tested by the immunisation of crossbred male calves at a dose of 200 microg/animal of each glycoprotein, three divided dosages. Following challenge infestations by the stages of two species of ticks, a significant rejection of (73.6%; P < 0.01) of larvae and adults (75.0%; P < 0.01) of H. a. anatolicum and adults (89.8%; P < 0.01) of B. microplus were noted. The direct effect of immunisation on the stages of the challenged ticks (%DT) was calculated as 69%, 52% against larvae and adults of H. a. anatolicum and 60% against adults of B. microplus. A direct corelation between protection and anti-glycoprotein antibody titre was detected. The effect of the isolated glycoproteins on limiting the tick infestation is discussed in detail.
在印度，控制蜱虫侵扰是高产杂交牛养殖户面临的一个主要问题。为了针对印度农民遇到的多蜱虫侵扰问题制定合适的免疫保护措施，人们尝试在小亚璃眼蜱和微小牛蜱的幼虫中鉴定候选蛋白分子。采用两步亲和层析法，分别从小亚璃眼蜱和微小牛蜱中分离出了34 kDa和29 kDa的糖蛋白。通过以200微克/动物的剂量、分三次给杂交雄性小牛注射每种糖蛋白来测试分离出的糖蛋白的相加效应。在受到两种蜱虫各阶段的攻击侵扰后，发现小亚璃眼蜱的幼虫和成虫（73.6%；P < 0.01）以及微小牛蜱的成虫（89.8%；P < 0.01）有显著的排斥反应。免疫对受攻击蜱虫各阶段的直接影响（%DT）计算得出，针对小亚璃眼蜱的幼虫和成虫分别为69%、52%，针对微小牛蜱的成虫为60%。检测到保护作用与抗糖蛋白抗体滴度之间存在直接相关性。详细讨论了分离出的糖蛋白在限制蜱虫侵扰方面的作用。
