Department of Pathology and Molecular Medicine, Queen's University and Kingston General Hospital, Kingston, Canada.
PLoS One. 2011;6(8):e22828. doi: 10.1371/journal.pone.0022828. Epub 2011 Aug 29.
MicroRNAs (miRNAs, miRs) are small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. MicroRNAs are dysregulated in cancer and may play essential roles in tumorigenesis. Additionally, miRNAs have been shown to have prognostic and diagnostic value in certain types of cancer. The objective of this study was to identify dysregulated miRNAs in endometrioid endometrial adenocarcinoma (EEC) and the precursor lesion, complex atypical hyperplasia (CAH).
We compared the expression profiles of 723 human miRNAs from 14 cases of EEC, 10 cases of CAH, and 10 normal proliferative endometria controls using Agilent Human miRNA arrays following RNA extraction from formalin-fixed paraffin-embedded (FFPE) tissues. The expression of 4 dysregulated miRNAs was validated using real time reverse transcription-PCR.
Forty-three miRNAs were dysregulated in EEC and CAH compared to normal controls (p<0.05). The entire miR-200 family (miR-200a/b/c, miR-141, and miR-429) was up-regulated in cases of EEC.
This information contributes to the candidate miRNA expression profile that has been generated for EEC and shows that certain miRNAs are dysregulated in the precursor lesion, CAH. These miRNAs in particular may play important roles in tumorigenesis. Examination of miRNAs that are consistently dysregulated in various studies of EEC, like the miR-200 family, will aid in the understanding of the role that miRNAs play in tumorigenesis in this tumour type.
微小 RNA（miRNAs，miRs）是一种小的非编码 RNA，可在转录后水平负调控基因表达。miRNAs 在癌症中失调，可能在肿瘤发生中发挥重要作用。此外，miRNAs 在某些类型的癌症中具有预后和诊断价值。本研究的目的是鉴定子宫内膜样腺癌（EEC）和前体病变复杂非典型增生（CAH）中失调的 miRNAs。
我们比较了 14 例 EEC、10 例 CAH 和 10 例正常增殖子宫内膜对照组织中 723 个人类 miRNA 的表达谱，采用 Agilent Human miRNA 微阵列，对福尔马林固定石蜡包埋（FFPE）组织中的 RNA 进行提取。采用实时逆转录-PCR 验证 4 个失调 miRNA 的表达。
与正常对照组相比，EEC 和 CAH 中有 43 个 miRNAs 失调（p<0.05）。整个 miR-200 家族（miR-200a/b/c、miR-141 和 miR-429）在 EEC 病例中上调。
这些信息为 EEC 生成的候选 miRNA 表达谱做出了贡献，并表明某些 miRNAs 在前体病变 CAH 中失调。这些 miRNA 可能在肿瘤发生中发挥重要作用。检查在各种 EEC 研究中一致失调的 miRNAs，如 miR-200 家族，将有助于了解 miRNA 在这种肿瘤类型的肿瘤发生中的作用。
