Ariën Kevin K, Vanham Guido, Gali Youssef
Virology Unit, Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium.
Curr Protoc Cell Biol. 2011 Sep;Chapter 26:Unit26.13. doi: 10.1002/0471143030.cb2613s52.
Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (FGT) is still under debate. Using epithelial cells originating from the FGT and primary cells as subepithelial HIV target cells, an in vitro dual-chamber model of the FGT was developed. Here we describe how this in vitro model can be used to assess the cellular toxicity and anti-HIV activity of antiretrovirals and formulations thereof that are intended to be used as microbicides.
人类免疫缺陷病毒（HIV）的异性传播是全球主要的感染方式。跨上皮感染的早期步骤至关重要，但HIV究竟如何在女性生殖道（FGT）中建立感染仍存在争议。利用源自女性生殖道的上皮细胞和作为上皮下HIV靶细胞的原代细胞，构建了一种女性生殖道的体外双室模型。在此，我们描述了如何利用该体外模型评估拟用作杀微生物剂的抗逆转录病毒药物及其制剂的细胞毒性和抗HIV活性。
