Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing, People's Republic of China.
Anal Chem. 2011 Nov 1;83(21):8245-51. doi: 10.1021/ac202039h. Epub 2011 Oct 3.
In this work, chemiluminescent (CL) property of the carbon dots in the presence of peroxynitrous acid was studied. Peroxynitrous acid is formed by online mixing of nitrite and acidified hydrogen peroxide. The CL intensity was increased linearly with nitrite concentration in the range from 1.0 × 10(-7) M to 1.0 × 10(-5) M, and the detection limit was 5.3 × 10(-8) M (signal-to-noise ratio of S/N = 3). This method has been successfully applied to the determination of nitrites in pond water, river water, and pure milk, with recoveries in the range of 98%-108%. The CL mechanism of the peroxynitrous acid-carbon dots system was investigated using the CL, ultraviolet-visible light (UV-vis), and electron paramagnetic resonance (EPR) spectra. The electron-transfer annihilation of hole-injected and electron-injected carbon dots could mainly account for the CL emission, which sheds new light on the optical properties of the carbon dots.
在这项工作中，研究了过亚硝酸酸存在下碳点的化学发光（CL）性质。过亚硝酸酸是由亚硝酸盐和酸化的过氧化氢在线混合形成的。CL 强度随亚硝酸盐浓度在线性范围内从 1.0×10(-7) M 增加到 1.0×10(-5) M，检测限为 5.3×10(-8) M（信噪比 S/N = 3）。该方法已成功应用于池塘水、河水和纯牛奶中亚硝酸盐的测定，回收率在 98%-108%范围内。通过 CL、紫外-可见光谱（UV-vis）和电子顺磁共振（EPR）谱研究了过亚硝酸酸-碳点体系的 CL 机制。空穴注入和电子注入碳点的电子转移湮灭可以主要解释 CL 发射，这为碳点的光学性质提供了新的认识。
