Institute of Traditional Medicine, National Yang-Ming University , Taipei, Taiwan, 11221.
J Agric Food Chem. 2011 Oct 12;59(19):10496-504. doi: 10.1021/jf202756f. Epub 2011 Sep 19.
Fisetin, a flavonoid compound commonly present in fruits and vegetables, can exert anti-inflammation activities via inhibition of the NF-κB-signaling pathway. This study aims to evaluate the antiasthma activity of fisetin and investigate its possible molecular mechanisms. We found that fisetin attenuated lung inflammation, goblet cell hyperplasia, and airway hyperresponsiveness in ovalbumin-induced asthma and decreased eosinophils and lymphocytes in bronchoalveolar lavage fluid. Fisetin treatment reduced expression of the key initiators of allergic airway inflammation (eotaxin-1 and TSLP), Th2-associated cytokines (IL-4, IL-5, and IL-13) in lungs, and Th2-predominant transcription factor GATA-3 and cytokines in thoracic lymph node cells and splenocytes. Notably, fisetin treatment impaired NF-κB activation in OVA-stimulated lung tissues and TNF-α-stimulated bronchial epithelial cells. Collectively, this study demonstrated the beneficial effect of fisetin in the amelioration of asthmatic phenotypes. The antiasthma activity of fisetin is associated with reduction of Th2 responses as well as suppression of NF-κB and its downstream chemokines.
漆黄素是一种常见于水果和蔬菜中的类黄酮化合物，可通过抑制 NF-κB 信号通路发挥抗炎作用。本研究旨在评估漆黄素的抗哮喘活性，并探讨其可能的分子机制。我们发现，漆黄素可减轻卵清蛋白诱导的哮喘中的肺部炎症、杯状细胞增生和气道高反应性，并减少支气管肺泡灌洗液中的嗜酸性粒细胞和淋巴细胞。漆黄素治疗可降低过敏性气道炎症的关键起始因子（eotaxin-1 和 TSLP）、Th2 相关细胞因子（IL-4、IL-5 和 IL-13）在肺中的表达，以及胸淋巴结细胞和脾细胞中的 Th2 优势转录因子 GATA-3 和细胞因子。值得注意的是，漆黄素治疗可损害 OVA 刺激的肺组织和 TNF-α 刺激的支气管上皮细胞中的 NF-κB 激活。总之，这项研究表明漆黄素在改善哮喘表型方面具有有益的作用。漆黄素的抗哮喘活性与减少 Th2 反应以及抑制 NF-κB 及其下游趋化因子有关。
