Department of Preventive & Social Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
Diabetol Metab Syndr. 2011 Sep 7;3(1):23. doi: 10.1186/1758-5996-3-23.
Epidemiological investigation of insulin resistance is difficult. Standard measures of insulin resistance require invasive investigations, which are impractical for large-scale studies. Surrogate measures using fasting blood samples have been developed, but even these are difficult to obtain in population-based studies. Measures of insulin resistance have not been validated in non-fasting blood samples. Our objective was to assess the correlations between fasting and non-fasting measures of insulin resistance/sensitivity.
Fasting and non-fasting measurements of metabolic function were compared in 30 volunteers (15 male) aged 28 to 48 years. Participants provided a morning blood sample after an overnight fast and a second sample approximately 4 hours after lunch on the same day.
Non-fasting levels of the adipokines leptin, adiponectin, and leptin:adiponectin ratios were not significantly different and highly correlated with fasting values (r values 0.95, 0.96, and 0.95 respectively, P values < 0.001). There were moderate correlations between fasting and non-fasting estimates of insulin sensitivity using the McAuley (r = 0.60, P = 0.001) and QUICKI formulae (r = 0.39, P = 0.037). The HOMA-IR estimate of insulin resistance was also moderately correlated (r = 0.45, P = 0.016).
Semi-fasting measures of leptin, adiponectin, and leptin:adiponectin ratios correlate closely with fasting values and are likely to be sufficient for population-based research. Other measures of insulin resistance or sensitivity in semi-fasted blood samples are moderately correlated with values obtained after an overnight fast. These estimates of insulin resistance/sensitivity may also be adequate for many epidemiological studies and would avoid the difficulties of obtaining fasting blood samples.
胰岛素抵抗的流行病学调查较为困难。标准的胰岛素抵抗测量方法需要进行侵入性检查，这在大规模研究中不切实际。虽然已经开发出了使用空腹血样的替代测量方法，但即使这些方法在基于人群的研究中也难以获得。在非空腹血样中尚未验证胰岛素抵抗的测量方法。我们的目的是评估空腹和非空腹胰岛素抵抗/敏感性测量之间的相关性。
对 30 名年龄在 28 至 48 岁的志愿者（15 名男性）进行了空腹和非空腹代谢功能测量。参与者在隔夜禁食后提供了早晨血样，并在当天午餐后大约 4 小时提供了第二份血样。
非空腹状态下的脂联素、瘦素和瘦素：脂联素比值等脂肪因子水平与空腹值无显著差异且高度相关（r 值分别为 0.95、0.96 和 0.95，P 值均＜0.001）。使用 McAuley（r = 0.60，P = 0.001）和 QUICKI 公式（r = 0.39，P = 0.037）对胰岛素敏感性的空腹和非空腹估计之间存在中度相关性。HOMA-IR 胰岛素抵抗估计值也呈中度相关（r = 0.45，P = 0.016）。
半空腹瘦素、脂联素和瘦素：脂联素比值的测量值与空腹值密切相关，对于基于人群的研究可能已经足够。在半空腹血样中测量的其他胰岛素抵抗或敏感性指标与隔夜禁食后获得的值中度相关。这些胰岛素抵抗/敏感性的估计值对于许多流行病学研究可能也是足够的，并且可以避免获取空腹血样的困难。
