D'Costa S, Yoon B-I, Kim D-Y, Motsinger-Reif A A, Williams M, Kim Y
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA.
Vet Pathol. 2012 Nov;49(6):971-8. doi: 10.1177/0300985811419529. Epub 2011 Sep 7.
The present study was performed to determine the morphologic change and selected molecular features of spontaneous lung tumors in cats examined at the North Carolina State University Veterinary Teaching Hospital. Thirty-nine primary lung carcinomas represented 0.69% of all feline cases admitted to the hospital. Most lung tumors were observed in aged cats (P < .0001), and no sex predilection was found (P < .4241). Persian cats with pulmonary carcinoma were overrepresented in the data set, at least 4 times more frequently than other breeds. The histologic tumor types included adenocarcinoma (64.1%), bronchioloalveolar carcinoma (20.5%), and adenosquamous carcinoma (15.4%). Metastasis was observed in about 80% of 39 cases, with decreasing order of intrapulmonary metastasis, intrathoracic carcinomatosis, regional lymph nodes, and distant organs, including digits. The size of the largest tumor mass was significantly associated with metastatic potential (P < .001). Based on immunohistochemistry, more than 80% (20 of 24) of feline lung tumors were positively labeled with either surfactant protein A or thyroid transcription factor 1. Epidermal growth factor receptor mutant and p53 proteins were detected in approximately 20% (5 of 24) and 25% (6 of 24) of the feline lung tumor cases, respectively. Limited sequencing analysis of K-ras and p53 genes in 3 selected normal and neoplastic lung tissues did not reveal any alteration. Results indicate that primary lung carcinomas are rare but aggressive tumors in cats, thereby warranting further studies on molecular carcinogenesis.
本研究旨在确定在北卡罗来纳州立大学兽医教学医院接受检查的猫自发性肺肿瘤的形态学变化和选定的分子特征。39例原发性肺癌占该医院收治的所有猫病例的0.69%。大多数肺肿瘤在老年猫中观察到（P <.0001），未发现性别偏好（P <.4241）。数据集中患有肺癌的波斯猫比例过高，至少比其他品种频繁4倍。组织学肿瘤类型包括腺癌（64.1%）、细支气管肺泡癌（20.5%）和腺鳞癌（15.4%）。在39例病例中约80%观察到转移，肺内转移、胸腔内癌转移、区域淋巴结和远处器官（包括趾部）的转移顺序递减。最大肿瘤块的大小与转移潜能显著相关（P <.001）。基于免疫组织化学，超过80%（24例中的20例）的猫肺肿瘤被表面活性蛋白A或甲状腺转录因子1阳性标记。在大约20%（24例中的5例）和25%（24例中的6例）的猫肺肿瘤病例中分别检测到表皮生长因子受体突变体和p53蛋白。对3个选定的正常和肿瘤性肺组织进行的K-ras和p53基因的有限测序分析未发现任何改变。结果表明，原发性肺癌在猫中罕见但具有侵袭性，因此有必要对分子致癌作用进行进一步研究。
