Cukor G, Blacklow N R, Capozza F E, Panjvani Z F, Bednarek F
J Clin Microbiol. 1979 Jan;9(1):93-6. doi: 10.1128/jcm.9.1.93-96.1979.
Human milk obtained from 21 American nursing mothers was studied for the presence of secretory immunoglobulin A antibody to rotavirus, the most common etiological agent of infantile gastroenteritis. Antibody was quantitated by adaptation of a recently described solid-phase radioimmunoassay technique that employs simian rotavirus as a convenient substitute antigen for human rotavirus. Of the mothers tested, 80% (12 of 15) possessed milk antibody within a week of parturition, whereas 56% of those tested (5 of 9) secreted milk antibody as late as 6 or 9 months postpartum. Specificity of the radioimmunoassay was demonstrated by absorption of antibody with purified rotavirus. Our detection by radioimmunoassay of antibody to rotavirus in milk samples collected past the colostrum stage is in contrast to other studies that have failed to detect antibody in human milk by immunofluorescence or neutralization tests. The present study also suggested that the appearance of secretory immunoglobulin A antibody in the milk of mothers previously lacking milk antibody may be correlated with subclinical infection of the mother with rotavirus.
对21位美国哺乳期母亲的母乳进行了研究，以检测其中针对轮状病毒的分泌型免疫球蛋白A抗体的存在情况，轮状病毒是婴儿肠胃炎最常见的病原体。通过采用一种最近描述的固相放射免疫测定技术来对抗体进行定量，该技术使用猴轮状病毒作为人轮状病毒的便捷替代抗原。在接受检测的母亲中，80%（15位中的12位）在分娩后一周内母乳中含有抗体，而56%的受测者（9位中的5位）在产后6个月或9个月时仍分泌母乳抗体。通过用纯化的轮状病毒吸收抗体，证明了放射免疫测定的特异性。我们通过放射免疫测定法在初乳期之后采集的母乳样本中检测到轮状病毒抗体，这与其他一些研究形成了对比，那些研究通过免疫荧光或中和试验未能在母乳中检测到抗体。本研究还表明，先前母乳中缺乏抗体的母亲，其母乳中分泌型免疫球蛋白A抗体的出现可能与母亲感染轮状病毒的亚临床感染有关。
