对人呼肠孤病毒样因子感染的免疫反应：采用酶联免疫吸附测定法检测抗人呼肠孤病毒样因子免疫球蛋白G和M水平
Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay.
作者信息
Yolken R H, Wyatt R G, Kim H W, Kapikian A Z, Chanock R M
出版信息
Infect Immun. 1978 Feb;19(2):540-6. doi: 10.1128/iai.19.2.540-546.1978.
The report describes the development of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against the human reovirus-like agent of infantile gastroenteritis (HRVLA). This ELISA system proved to be four times as sensitive as the standard anti-HRVLA fluorescent-antibody assay and ten times as sensitive as the standard anti-HRVLA complement fixation assay. In addition, the ELISA was capable of determining immunoglobulin G (IgG) and IgM subclasses of anti-HRVLA antibody using a single dilution os serum. With this assay, it was discovered that 11 of 21 infected children had anti-HRVLA IgM in their acute sera before the appearance of anti-HRVLA IgG. ELISA is a useful tool in the evaluation of immunological response to HRVLA infection.
该报告描述了一种用于检测抗婴儿肠胃炎人呼肠孤病毒样病原体（HRVLA）抗体的酶联免疫吸附测定（ELISA）的开发。这种ELISA系统被证明比标准抗HRVLA荧光抗体测定法灵敏四倍，比标准抗HRVLA补体结合测定法灵敏十倍。此外，ELISA能够使用单一稀释度的血清测定抗HRVLA抗体的免疫球蛋白G（IgG）和IgM亚类。通过该测定发现，21名受感染儿童中有11名在出现抗HRVLA IgG之前，其急性期血清中就有抗HRVLA IgM。ELISA是评估对HRVLA感染免疫反应的有用工具。
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