[溶菌酶制剂溶菌酰胺酶对金黄色葡萄球菌209P细胞的作用]
[The effect of bacteriolytic preparation lysoamidase on Staphylococcus aureus 209P cells].
作者信息
Abramochkin G V, Ratner E N, Petrov V V, Severin A I, Suzina N E, Fikhte B A, Kulaev I S
出版信息
Prikl Biokhim Mikrobiol. 1990 Jan-Feb;26(1):93-100.
The effect of the bacteriolytic preparation "Lysoamidase" on Staphylococcus aureus 299 P was studied. The maximum activity of the preparation was observed at pH 8.0 ionic strength 0.01-0.02 M and 50-60 degrees of the incubation medium. The electron microscopic examination revealed that "Lysoamidase" hydrolyzed the cell wall in one or several points with the following osmotic shock and extrusion of the cytoplasm. In an isotonic solution (1 M sucrose) "Lysoamidase" caused protoplast formation.
研究了溶菌制剂“溶菌酰胺酶”对金黄色葡萄球菌299P的作用。在pH 8.0、离子强度0.01 - 0.02 M以及培养介质温度50 - 60度时观察到该制剂的最大活性。电子显微镜检查显示，“溶菌酰胺酶”在细胞壁的一点或几点水解细胞壁，随后发生渗透休克和细胞质挤出。在等渗溶液（1 M蔗糖）中，“溶菌酰胺酶”导致原生质体形成。
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