[The components of the microbial preparation of lysoamidase from the Pseudomonodaceae family responsible for its bacteriolytic activity].

The contribution of enzymes isolated from the microbial enzymic preparation to its total bacteriolytic activity was studied. The combined action of the lytic proteinase L2 and the lytic fraction L1 used in the same ratio as in the lysoamidase preparation resulted in a complete recovery of the bacteriolytic activity. During a 4-fold increase of the proportion of the lytic enzyme L1 as compared with lytic proteinase L2, the activity of the reconstituted preparation increased by 64%. Neutral phosphomonohydrolase, metal proteinase and the polysaccharide isolated from the lysoamidase preparation had no effect on the bacteriolytic activity of the reconstituted preparation. The polysaccharide isolated from lysoamidase increased the thermal stability of the preparation obtained up to that of lysoamidase.