Department of Molecular Virology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822 GDANSK, Poland.
Curr Pharm Biotechnol. 2011 Nov;12(11):1840-9. doi: 10.2174/138920111798377012.
A major advantage of recombinant DNA technology is its flexibility allowing for "on demand" production of specific proteins with theurapeutic value in heterologous expression systems. Gene expression vectors based on baculovirus, insect virus attacking mostly lepidopteran species, are frequently used for relatively inexpensive and fast production of such proteins. This expression system is recognized as one of the most powerful technologies for commercial synthesis of glycoproteins originating from vertebrate themselves or from vertebrate viruses. Glycosylation pathways utilized by insects are not identical, though they are similar to vertebrate glycosylation pathways. In the review special attention is given to the development of new virus-like particles (VLPs) potential vaccines which represent a novel class of subunit vaccines that are able to stimulate efficiently cellular and humoral immune responses against viral agents. Apart from production of vertebrate proteins or VLPs "on demand " in insect cells, a new exciting field of using baculovirus as gene delivery system to vertebrate cells was recently open which has a great potential for future uses of baculovirus as effective gene therapy vector.
重组 DNA 技术的一个主要优势在于其灵活性，允许在异源表达系统中“按需”生产具有治疗价值的特定蛋白质。基于杆状病毒的基因表达载体，是一种攻击鳞翅目物种的昆虫病毒，常用于相对廉价且快速生产此类蛋白质。这种表达系统被认为是最强大的技术之一，可用于商业合成来源于脊椎动物自身或脊椎动物病毒的糖蛋白。尽管昆虫使用的糖基化途径与脊椎动物的糖基化途径相似，但并不完全相同。在综述中，特别关注了新型病毒样颗粒（VLPs）疫苗的开发，这类新型亚单位疫苗能够有效地刺激针对病毒的细胞和体液免疫反应。除了在昆虫细胞中“按需”生产脊椎动物蛋白或 VLPs 之外，最近还开辟了一个新的研究领域，即将杆状病毒用作基因传递系统向脊椎动物细胞传递基因，这为未来利用杆状病毒作为有效的基因治疗载体提供了巨大的潜力。
