Yamaji Hideki, Konishi Eiji
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
BIKEN Endowed Department of Dengue Vaccine Development, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
Methods Mol Biol. 2016;1404:365-375. doi: 10.1007/978-1-4939-3389-1_25.
Virus-like particles (VLPs) can be produced via the expression of virus surface proteins that self-assemble into particulate structures in recombinant protein expression systems. Expression of the DNA fragment encoding the Japanese encephalitis (JE) virus prM signal peptide, the precursor (prM) of the viral membrane protein (M), and the envelope glycoprotein (E) allows the production of a secretory form of VLPs. Expression systems that use lepidopteran insect cells, such as the baculovirus-insect cell system and stably transformed insect cells, can be used for the efficient production of JE VLPs. This chapter describes the production of JE VLPs from stably transformed lepidopteran insect cells.
病毒样颗粒（VLPs）可通过在重组蛋白表达系统中表达能自组装成颗粒结构的病毒表面蛋白来产生。编码日本脑炎（JE）病毒prM信号肽、病毒膜蛋白（M）的前体（prM）和包膜糖蛋白（E）的DNA片段的表达可产生分泌形式的VLPs。使用鳞翅目昆虫细胞的表达系统，如杆状病毒-昆虫细胞系统和稳定转化的昆虫细胞，可用于高效生产JE VLPs。本章描述了从稳定转化的鳞翅目昆虫细胞中生产JE VLPs的方法。
