Institute for Biological Sciences, National Research Council Canada, Ottawa, Canada.
Carbohydr Res. 2011 Nov 8;346(15):2437-44. doi: 10.1016/j.carres.2011.06.036. Epub 2011 Jul 13.
We describe a re-investigation of the structure of the lipopolysaccharide (LPS) from Helicobacter pylori genomic strain 26695 and its corresponding HP0826::Kan mutant lacking the O-chain component based on the in-depth NMR analysis of the oligosaccharide products obtained through the use of various degradation procedures performed on the purified LPS from both strains, as well as CE-MS data. New structural evidence indicates the presence of the linear arrangement of glucan and heptan portions of the LPS attached through -6-α-DDHep-3-α-L-Fuc-3-β-GlcNAc- fragment to the inner core DD-heptose residue. This structure differs from previously reported structures of the H. pylori 26695 LPS in several aspects.
我们基于对来自幽门螺旋杆菌基因组菌株 26695 的脂多糖（LPS）及其相应的 HP0826::Kan 突变体（缺乏 O-链成分）的深入 NMR 分析，以及 CE-MS 数据，对其结构进行了重新研究。这些结果是基于对两种 LPS 进行各种降解处理后获得的寡糖产物进行的深入 NMR 分析以及 CE-MS 数据得出的。新的结构证据表明，LPS 的葡聚糖和庚烷部分通过 -6-α-DDHep-3-α-L-Fuc-3-β-GlcNAc- 片段连接到内部核心 DD-庚糖残基，呈线性排列。该结构与之前报道的幽门螺旋杆菌 26695 LPS 结构在几个方面存在差异。
