Altman Eleonora, Chandan Vandana, Larocque Suzon, Aubry Annie, Logan Susan M, Vinogradov Evgeny, Li Jianjun
Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, Canada.
FEMS Immunol Med Microbiol. 2008 Jul;53(2):204-13. doi: 10.1111/j.1574-695X.2008.00416.x. Epub 2008 Apr 21.
The outer core region of Helicobacter pylori lipopolysaccharide of the majority of isolates contains an alpha-1,6-glucan polymer synthesized by the product of the HP0159 ORF. Structural studies carried out on HP0159 lipopolysaccharide mutants by a combination of chemical methods, mass spectrometry and nuclear magnetic resonance spectroscopy confirmed that insertional inactivation of HP0159 gene in H. pylori strains 26695 and SS1 resulted in formation of a truncated lipopolysaccharide molecule characterized by the presence of a terminal dd-heptose residue in the side-chain outer core fragment and maintaining an inner core backbone structure compared with the wild-type Lewis antigen-expressing strains. Colonization studies with HP0159 mutants of two mouse-colonizing strains, SS1 and M6, confirmed their inability to successfully colonize the murine stomach.
大多数幽门螺杆菌分离株的脂多糖外核心区域含有由HP0159开放阅读框产物合成的α-1,6-葡聚糖聚合物。通过化学方法、质谱和核磁共振光谱相结合对HP0159脂多糖突变体进行的结构研究证实，幽门螺杆菌菌株26695和SS1中HP0159基因的插入失活导致形成截短的脂多糖分子，其特征是侧链外核心片段中存在末端dd-庚糖残基，并且与表达野生型Lewis抗原的菌株相比，保持内核心主链结构。对两种定殖于小鼠的菌株SS1和M6的HP0159突变体进行的定殖研究证实，它们无法成功定殖于小鼠胃部。
