Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
J Med Microbiol. 2012 Jan;61(Pt 1):126-131. doi: 10.1099/jmm.0.035477-0. Epub 2011 Sep 8.
A total of 143 group B streptococcus (GBS) isolates collected from mothers at the Maternity Hospital in Kuwait were investigated for their serotypes and antibiotic resistance, and screened by PCR for the carriage of genes for resistance to tetracycline (tetk, tetM, tetL, tetO), erythromycin (ermA, ermB, ermC, ermTR, ermM, mefA, mefE, msrA) and aminoglycosides (aph3, ant4, ant6). All isolates were serotyped using a latex agglutination test. Most of the isolates belonged to serotypes V (38.5 %), III (20.9 %), Ia (7.7 %) and II (11.2 %). Sixteen isolates (11.2 %) were nontypable. All isolates were susceptible to penicillin, ampicillin and cefotaxime (MICs 0.016-0.094 µg ml(-1)) but were resistant to trimethoprim (92.3 %), tetracycline (89.5 %), minocycline (89.5 %), high-level kanamycin (76.9 %), chloramphenicol (30.0 %), erythromycin (12.6 %), clindamycin (7.0 %), high-level streptomycin (3.5 %) and ciprofloxacin (0.7 %). The tetracycline-resistant isolates contained tetM (94.5 %), tetO (3.9 %), tetL (1.6 %) and tetK (0.8 %). The erythromycin-resistant isolates contained ermB (61.1 %), ermTR (38.9 %), ermA (5.5 %), mefA (5.5 %) and mefE (11 %). All high-level kanamycin-resistant isolates contained aph3. One of the high-level streptomycin-resistant isolates contained ant6. Partial DNA sequencing of aph3 revealed sequences with 99 % similarity to aph3 found in Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus and Staphylococcus epidermidis, suggesting that the GBS isolates could have acquired aph3 from other Gram-positive cocci. The high proportion of isolates with resistance to tetracycline, high-level kanamycin and trimethoprim, and the increase in the prevalence of erythromycin resistance, represents an emerging public health concern that needs further surveillance.
总共从科威特妇产医院的母亲身上采集了 143 株 B 群链球菌（GBS）分离株，对其血清型和抗生素耐药性进行了调查，并通过 PCR 筛选了对四环素（tetk、tetM、tetL、tetO）、红霉素（ermA、ermB、ermC、ermTR、ermM、mefA、mefE、msrA）和氨基糖苷类（aph3、ant4、ant6）耐药的基因。所有分离株均使用乳胶凝集试验进行血清型鉴定。大多数分离株属于血清型 V（38.5%）、III（20.9%）、Ia（7.7%）和 II（11.2%）。16 株（11.2%）分离株为非定型。所有分离株均对青霉素、氨苄西林和头孢噻肟敏感（MIC 值为 0.016-0.094 µg ml(-1)），但对甲氧苄啶（92.3%）、四环素（89.5%）、米诺环素（89.5%）、高水平卡那霉素（76.9%）、氯霉素（30.0%）、红霉素（12.6%）、克林霉素（7.0%）、高水平链霉素（3.5%）和环丙沙星（0.7%）耐药。四环素耐药株含有 tetM（94.5%）、tetO（3.9%）、tetL（1.6%）和 tetK（0.8%）。红霉素耐药株含有 ermB（61.1%）、ermTR（38.9%）、ermA（5.5%）、mefA（5.5%）和 mefE（11%）。所有高水平卡那霉素耐药株均含有 aph3。1 株高水平链霉素耐药株含有 ant6。aph3 的部分 DNA 测序显示，其序列与粪肠球菌、屎肠球菌、金黄色葡萄球菌和表皮葡萄球菌中发现的 aph3 具有 99%的相似性，这表明 GBS 分离株可能从其他革兰阳性球菌获得了 aph3。四环素、高水平卡那霉素和甲氧苄啶耐药分离株的比例较高，以及红霉素耐药性的增加，这代表了一个需要进一步监测的新出现的公共卫生问题。
