Department of Botany, Graduate School of Science, Kyoto University, Kyoto, Japan.
Plant Signal Behav. 2011 Oct;6(10):1454-6. doi: 10.4161/psb.6.10.17344. Epub 2011 Oct 1.
The production of transgenic plants has contributed greatly to plant research. Previously, an improved method for screening transgenic Arabidopsis thaliana seeds using the FAST (Fluorescence-Accumulating-Seed Technology) method and FAST marker was reported. Arabidopsis seeds containing the FAST marker may be visually screened using a fluorescence stereomicroscope or blue LED handy-type instrument. Although the FAST method was originally designed for Arabidopsis screens, this study endeavors to adapt this method for the screening of other plants. Here, an optimized technology, designated the OsFAST method, is presented as a useful tool for screening transgenic rice seeds. The OsFAST method is based on the expression of the OsFAST-G marker under the control of a seed-embryo-specific promoter, similar to the Arabidopsis FAST-G marker. The OsFAST method provides a simple and non-destructive method for identifying transgenic rice seeds. It is proposed that the FAST method is adaptable to various plant species and will enable a deeper analysis of the floral-dip method.
转基因植物的生产极大地促进了植物研究。先前，已经报道了一种使用 FAST（荧光累积种子技术）方法和 FAST 标记物来筛选转基因拟南芥种子的改良方法。含有 FAST 标记物的拟南芥种子可以使用荧光立体显微镜或蓝色 LED 手持式仪器进行目视筛选。尽管 FAST 方法最初是为拟南芥筛选设计的，但本研究旨在将该方法适应于其他植物的筛选。在这里，提出了一种优化的技术，称为 OsFAST 方法，作为筛选转基因水稻种子的有用工具。OsFAST 方法基于在种子-胚胎特异性启动子的控制下表达 OsFAST-G 标记物，类似于拟南芥 FAST-G 标记物。OsFAST 方法提供了一种简单且非破坏性的方法来鉴定转基因水稻种子。提出 FAST 方法可适应于各种植物物种，并将使花浸法的分析更加深入。
