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病毒感染期间自然杀伤细胞中穿孔素的体内表达。关于单纯疱疹病毒I型引起的葡萄膜炎的研究。

In vivo expression of perforin by natural killer cells during a viral infection. Studies on uveitis produced by herpes simplex virus type I.

作者信息

Young L H, Foster C S, Young J D

机构信息

Retina Service, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston.

出版信息

Am J Pathol. 1990 May;136(5):1021-30.

PMID:2190470
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1877436/
Abstract

A potent cytolytic pore-forming protein (PFP, perforin, or cytolysin) is associated with the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. The role of PFP/perforin in cytolytic reactions carried out in vivo is still unclear. Here, the authors performed immunohistochemical analysis using antibodies monospecific for perforin and made use of a murine uveitis model produced by intracameral inoculation of herpes simplex virus I (HSV-I). The main cell infiltrate found in the anterior segment of virus-inoculated eyes consisted of Thy-1+/asialo GM1+/CD8-/CD4- cells, presumably representing NK cells. Perforin staining was detected mainly in cells bearing this phenotype. Perforin was only detected in cells displaying the large granular lymphocyte morphology. A small number of perforin-positive cells (less than 5%) colabeled as CD8+, indicating that these cells could have belonged to the CTL lineage. These observations show for the first time the presence of perforin-containing NK cells in tissues of animals undergoing acute viral infections.

摘要

一种强效的细胞溶解性成孔蛋白（PFP，穿孔素，或溶细胞素）与细胞毒性T淋巴细胞（CTL）和自然杀伤（NK）细胞的细胞质颗粒相关。PFP/穿孔素在体内进行的细胞溶解反应中的作用仍不清楚。在这里，作者使用对穿孔素具有单特异性的抗体进行免疫组织化学分析，并利用通过前房接种单纯疱疹病毒I（HSV-I）产生的小鼠葡萄膜炎模型。在接种病毒的眼睛前房中发现的主要细胞浸润由Thy-1+/去唾液酸GM1+/CD8-/CD4-细胞组成，推测代表NK细胞。穿孔素染色主要在具有这种表型的细胞中检测到。穿孔素仅在显示大颗粒淋巴细胞形态的细胞中检测到。少数穿孔素阳性细胞（少于5%）共标记为CD8+，表明这些细胞可能属于CTL谱系。这些观察首次表明在经历急性病毒感染的动物组织中存在含穿孔素的NK细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ef9/1877436/596eb4e2d3f7/amjpathol00113-0045-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ef9/1877436/c57996d9c660/amjpathol00113-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ef9/1877436/9746c184c763/amjpathol00113-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ef9/1877436/344760f67416/amjpathol00113-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ef9/1877436/b95af080ef9d/amjpathol00113-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ef9/1877436/7d67dad8a590/amjpathol00113-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ef9/1877436/c749bfc82dd5/amjpathol00113-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ef9/1877436/596eb4e2d3f7/amjpathol00113-0045-b.jpg

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本文引用的文献

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In vivo effects of anti-asialo GM1. I. Reduction of NK activity and enhancement of transplanted tumor growth in nude mice.抗去唾液酸GM1的体内效应。I. 裸鼠自然杀伤细胞活性降低及移植瘤生长增强
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Natural killer cells: their roles in defenses against disease.自然杀伤细胞：它们在抵御疾病中的作用。
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Establishment of monoclonal anti-Nk-1.1 antibody.抗Nk-1.1单克隆抗体的制备
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Monoclonal antibodies distinguish subtypes of retinal horizontal cells.单克隆抗体可区分视网膜水平细胞的亚型。
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Cytotoxic T-lymphocytes. How do they function?细胞毒性T淋巴细胞。它们是如何发挥作用的？
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Correlation between low natural killing of fibroblasts infected with herpes simplex virus type 1 and susceptibility to herpesvirus infections.感染1型单纯疱疹病毒的成纤维细胞自然杀伤率低与疱疹病毒感染易感性之间的相关性。
J Infect Dis. 1983 Jun;147(6):1030-5. doi: 10.1093/infdis/147.6.1030.
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Human natural killer cells: biologic and pathologic aspects.人类自然杀伤细胞：生物学与病理学方面。
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Relationship between expression of herpes simplex virus glycoproteins and susceptibility of target cells to human natural killer activity.单纯疱疹病毒糖蛋白的表达与靶细胞对人自然杀伤活性的敏感性之间的关系。
J Exp Med. 1983 May 1;157(5):1544-61. doi: 10.1084/jem.157.5.1544.
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