Hameed A, Olsen K J, Cheng L, Fox W M, Hruban R H, Podack E R
Department of Pathology, Johns Hopkins Hospital, Baltimore, Maryland 21205.
Am J Pathol. 1992 May;140(5):1025-30.
Perforin is a potent cytolytic pore-forming protein expressed in cytoplasmic granules of cytotoxic T lymphocytes and natural killer cells. A new monoclonal antibody raised against human perforin was used to detect both in vitro and in vivo perforin expression in cytotoxic cells. Immunohistochemical analysis of human peripheral blood mononuclear cells cultured in recombinant interleukin-2 (rIL-2) showed strong granular cytoplasmic staining of the IL-2 activated cytotoxic cells. Fresh-frozen tissue sections from patients with heart allograft rejection were also stained. Strong granular cytoplasmic staining of the mononuclear inflammatory infiltrate characteristic for perforin in cardiac allograft rejection was observed. The detection and quantitative analysis of perforin-associated cytotoxic cells by the human anti-perforin monoclonal antibody will help to evaluate the significance of these functionally distinct cytotoxic cells in human tissue.
穿孔素是一种在细胞毒性T淋巴细胞和自然杀伤细胞的细胞质颗粒中表达的强效溶细胞成孔蛋白。一种新的抗人穿孔素单克隆抗体被用于检测细胞毒性细胞在体外和体内的穿孔素表达。对在重组白细胞介素-2（rIL-2）中培养的人外周血单个核细胞进行免疫组织化学分析，结果显示IL-2激活的细胞毒性细胞有强烈的颗粒状细胞质染色。对心脏同种异体移植排斥患者的新鲜冷冻组织切片也进行了染色。观察到心脏同种异体移植排斥中单核炎性浸润的穿孔素特征性强烈颗粒状细胞质染色。用人抗穿孔素单克隆抗体对穿孔素相关细胞毒性细胞进行检测和定量分析，将有助于评估这些功能不同的细胞毒性细胞在人体组织中的意义。
