Department of Microbiology and Immunology, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Republic of Korea.
Nat Nanotechnol. 2011 Sep 11;6(10):675-82. doi: 10.1038/nnano.2011.149.
Dendritic cell-based cancer immunotherapy requires tumour antigens to be delivered efficiently into dendritic cells and their migration to be monitored in vivo. Nanoparticles have been explored as carriers for antigen delivery, but applications have been limited by the toxicity of the solvents used to make nanoparticles, and by the need to use transfection agents to deliver nanoparticles into cells. Here we show that an iron oxide-zinc oxide core-shell nanoparticle can deliver carcinoembryonic antigen into dendritic cells while simultaneously acting as an imaging agent. The nanoparticle-antigen complex is efficiently taken up by dendritic cells within one hour and can be detected in vitro by confocal microscopy and in vivo by magnetic resonance imaging. Mice immunized with dendritic cells containing the nanoparticle-antigen complex showed enhanced tumour antigen specific T-cell responses, delayed tumour growth and better survival than controls.
基于树突状细胞的癌症免疫疗法需要将肿瘤抗原有效地递送到树突状细胞中，并监测其在体内的迁移。纳米粒子已被探索作为抗原递送的载体，但由于用于制造纳米粒子的溶剂的毒性以及需要使用转染试剂将纳米粒子递送到细胞中，其应用受到限制。在这里，我们表明，氧化铁-氧化锌核壳纳米粒子可以在将癌胚抗原递送到树突状细胞的同时充当成像剂。纳米粒子-抗原复合物在一小时内被树突状细胞有效摄取，并且可以通过共聚焦显微镜在体外和通过磁共振成像在体内检测到。用含有纳米粒子-抗原复合物的树突状细胞免疫的小鼠表现出增强的肿瘤抗原特异性 T 细胞反应、延迟的肿瘤生长和更好的生存比对照组。
