WCU Hybrid Materials Program, Department of Materials Science and Engineering, Seoul National University, Seoul, 151-744, Korea.
J Mater Sci Mater Med. 2011 Nov;22(11):2437-47. doi: 10.1007/s10856-011-4431-3. Epub 2011 Sep 10.
Hydroxyapatite (HA) was coated onto pure magnesium (Mg) with an MgF(2) interlayer in order to reduce the surface corrosion rate and enhance the biocompatibility. Both MgF(2) and HA were successfully coated in sequence with good adhesion properties using the fluoride conversion coating and aerosol deposition techniques, respectively. In a simulated body fluid (SBF), the double layer coating remarkably enhanced the corrosion resistance of the coated Mg specimen. The in vitro cellular responses of the MC3T3-E1 pre-osteoblasts were examined using a cell proliferation assay and an alkaline phosphatase (ALP) assay, and these results demonstrated that the double coating layer also enhanced cell proliferation and differentiation levels. In the in vivo study, the HA/MgF(2) coated Mg corroded less than the bare Mg and had a higher bone-to-implant contact (BIC) ratio in the cortical bone area of the rabbit femora 4 weeks after implantation. These in vitro and in vivo results suggested that the HA coated Mg with the MgF(2) interlayer could be used as a potential candidate for biodegradable implant materials.
为了降低表面腐蚀速率并提高生物相容性，在纯镁（Mg）表面涂覆了羟基磷灰石（HA），中间夹有一层氟化镁（MgF2）作为过渡层。分别采用氟化物转化涂层和喷雾沉积技术，成功地依次涂覆了 MgF2和 HA，且具有良好的附着力。在模拟体液（SBF）中，双层涂层显著提高了涂层 Mg 试样的耐腐蚀性。通过细胞增殖试验和碱性磷酸酶（ALP）试验检测 MC3T3-E1 前成骨细胞的体外细胞反应，结果表明双层涂层还能提高细胞增殖和分化水平。在体内研究中，植入后 4 周，HA/MgF2涂层的 Mg 腐蚀程度低于裸 Mg，且在兔股骨皮质骨区域的骨与植入物接触（BIC）比率更高。这些体外和体内结果表明，具有 MgF2过渡层的 HA 涂层 Mg 可用作可生物降解植入材料的潜在候选材料。
