Institute of Materials Research, Division for Metallic Biomaterials, Helmholtz-Zentrum Geesthacht (HZG), 21502 Geesthacht, Germany.
Department of Medical Sciences, University of Ferrara, 44121 Ferrara, Italy.
Int J Mol Sci. 2020 Jul 19;21(14):5099. doi: 10.3390/ijms21145099.
Osteosarcoma is among the most common cancers in young patients and is responsible for one-tenth of all cancer-related deaths in children. Surgery often leads to bone defects in excised tissue, while residual cancer cells may remain. Degradable magnesium alloys get increasing attention as orthopedic implants, and some studies have reported potential antitumor activity. However, most of the studies do not take the complex interaction between malignant cells and their surrounding stroma into account. Here, we applied a coculture model consisting of green fluorescent osteosarcoma cells and red fluorescent fibroblasts on extruded Mg and Mg-6Ag with a tailored degradation rate. In contrast to non-degrading Ti-based material, both Mg-based materials reduced relative tumor cell numbers. Comparing the influence of the material on a sparse and dense coculture, relative cell numbers were found to be statistically different, thus relevant, while magnesium alloy degradations were observed as cell density-independent. We concluded that the sparse coculture model is a suitable mechanistic system to further study the antitumor effects of Mg-based material.
骨肉瘤是青少年中最常见的癌症之一，占儿童癌症相关死亡人数的十分之一。手术常常导致切除组织中的骨缺损，而残留的癌细胞可能仍然存在。可降解镁合金作为骨科植入物越来越受到关注，一些研究报告了其潜在的抗肿瘤活性。然而，大多数研究都没有考虑到恶性细胞与其周围基质之间的复杂相互作用。在这里，我们应用了一种共培养模型，该模型由绿色荧光骨肉瘤细胞和红色荧光成纤维细胞组成，共培养在挤出的 Mg 和具有定制降解率的 Mg-6Ag 上。与非降解的 Ti 基材料相比，两种 Mg 基材料均减少了相对肿瘤细胞数量。比较材料对稀疏和密集共培养的影响，发现相对细胞数量在统计学上有显著差异，因此是相关的，而镁合金降解则与细胞密度无关。我们得出结论，稀疏共培养模型是一个合适的机制系统，可以进一步研究 Mg 基材料的抗肿瘤作用。
