Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Neurochem Res. 2012 Jan;37(1):126-33. doi: 10.1007/s11064-011-0590-3. Epub 2011 Sep 11.
This study was carried out to ascertain the effects of maternal separation (3 h per day) of mothers from their pups in the neonatal period in rats, which has been suggested to induce a depressive-like state, would have long lasting effects on different parameters including hippocampal Na(+), K(+)-ATPase activity, NO production, free radical production and antioxidant enzymes activities in dams. Fourty-eight Wistar rats were divided into 3 groups: control, brief separation (10 min) and long separation (3 h). The neonatal interventions were done on postpartum days 1-10. At 35 days post-partum the dams were killed and the hippocampal Na(+), K(+)-ATPase activity were measured, as well as the activity of the antioxidant enzymes catalase, glutathione peroxidase, superoxide dismutase, free radicals production, and the production of nitric oxide. Hippocampal Na(+), K(+)-ATPase activity was decreased in the brief separated group and in dams subjected to 3 h separation from their pups. A reduction in nitric oxide levels in the hippocampus in dams of the long separated group was also observed. It is concluded that the withdrawal of pups from their mothers make the mothers more susceptible to the development of neurochemical alterations that could be related to depressive features.
本研究旨在确定在新生期将母亲与幼崽分离（每天 3 小时）对大鼠的影响，这种分离被认为会导致类似抑郁的状态，并对包括海马 Na(+)、K(+)-ATP 酶活性、NO 产生、自由基产生和母鼠抗氧化酶活性在内的不同参数产生长期影响。48 只 Wistar 大鼠被分为 3 组：对照组、短暂分离（10 分钟）和长期分离（3 小时）。新生儿干预在产后第 1-10 天进行。产后 35 天处死母鼠，测量海马 Na(+)、K(+)-ATP 酶活性以及抗氧化酶过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶的活性、自由基产生和一氧化氮的产生。短暂分离组和与幼崽分离 3 小时的母鼠的海马 Na(+)、K(+)-ATP 酶活性降低。还观察到长期分离组母鼠海马中一氧化氮水平降低。研究结果表明，幼崽与母亲分离会使母亲更容易发生神经化学改变，这可能与抑郁特征有关。
