Huang Guan-Cheng, Chen Shih-Yu, Tsai Po-Wei, Ganzon Jerome G, Lee Chia-Jung, Shiah Her-Shyong, Wang Ching-Chiung
School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Division of Hematology and Oncology, Department of Internal Medicine, Yuan's General Hospital, Fooyin University, Kaohsiung, Taiwan; Department of Leisure and Recreation Industry Management, College of Humanities and Management, Fooyin University, Kaohsiung, Taiwan.
Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan.
Drug Des Devel Ther. 2016 Mar 2;10:949-57. doi: 10.2147/DDDT.S94309. eCollection 2016.
Dang-Gui-Bu-Xue-Tang (DBT), a combination of Angelicae Sinensis Radix and Astragali Radix, is a widely used herbal decoction in traditional Chinese medicine primarily to promote or invigorate the "blood". In this study, we explored this ancient formulation and provide evidence of its blood-toning properties. We used the improvement iron uptake as promote or invigorate the "blood" indicator. Ferritin formation of Caco-2 cells in vitro assay and diet-induced anemia (DIA) in rat model were used to prove its improvement iron uptake and ameliorating effects. Finally, the iron-DBT interactions were measured by iron-binding assay. We first demonstrated DBT increased uptake of ferrous iron through the biosynthesis of ferritin by Caco-2 cells and determined which complementary treatment would provide optimum results. Thereafter, effects of the treatment on improving the bioavailability of absorbed iron in the form of hemoglobin (Hb) were established using a DIA-animal model. The results showed that DBT slightly improved Hb levels compared with the baseline Hb and pretreatment with DBT for 2 hours prior to supplementation with ferrous sulfate provided the greatest gain in Hb levels in DIA rats. However, DBT and ferrous sulfate were co-treated with Caco-2 cell or DIA rats, the ferritin formation and Hb levels both were decreased. In iron-binding assay, the DBT extract influenced the free Fe(II) type in the FeSO4 solution. Therefore, we suggest that complementary treatment with DBT and iron supplementation can have a strong ameliorating effect on iron-deficiency anemia in clinical settings, but needs a 2-hour interval of DBT administration prior to ferrous sulfate treatment.
当归补血汤（DBT）由当归和黄芪组成，是一种在传统中医中广泛使用的草药汤剂，主要用于促进或补益“血”。在本研究中，我们探究了这一古老配方，并提供了其补血特性的证据。我们将改善铁吸收作为促进或补益“血”的指标。采用体外培养的Caco-2细胞铁蛋白形成实验和大鼠饮食性贫血（DIA）模型来证明其改善铁吸收和改善贫血的作用。最后，通过铁结合实验测定铁与DBT的相互作用。我们首先证明DBT通过Caco-2细胞铁蛋白的生物合成增加亚铁的吸收，并确定哪种联合治疗能提供最佳效果。此后，使用DIA动物模型确定该治疗对提高以血红蛋白（Hb）形式吸收的铁的生物利用度的作用。结果表明，与基线Hb相比，DBT能轻微提高Hb水平，在补充硫酸亚铁前2小时用DBT预处理能使DIA大鼠的Hb水平提高最多。然而，将DBT和硫酸亚铁联合处理Caco-2细胞或DIA大鼠时，铁蛋白形成和Hb水平均降低。在铁结合实验中，DBT提取物影响了硫酸亚铁溶液中的游离Fe(II)类型。因此，我们认为在临床环境中，DBT与铁补充剂联合治疗对缺铁性贫血有很强的改善作用，但在硫酸亚铁治疗前需要间隔2小时给予DBT。
