Fraunhofer Institute of Biomedical Engineering, St. Ingbert, Germany.
Cryobiology. 2011 Dec;63(3):175-85. doi: 10.1016/j.cryobiol.2011.06.003. Epub 2011 Sep 3.
Human embryonic stem cells (hESCs) are candidates for many applications in the areas of regenerative medicine, tissue engineering, basic scientific research as well as pharmacology and toxicology. However, use of hESCs is limited by their sensitivity to freezing and thawing procedures. Hence, this emerging science needs new, reliable preservation methods for the long-term storage of large quantities of functional hESCs remaining pluripotent after post-thawing and culturing. Here, we present a highly efficient, surface based vitrification method for the cryopreservation of large numbers of adherent hESC colonies, using modified cell culture substrates. This technique results in much better post-thaw survival rate compared to cryopreservation in suspension and allows a quick and precise handling and storage of the cells, indicating low differentiation rates.
人胚胎干细胞（hESCs）在再生医学、组织工程、基础科学研究以及药理学和毒理学等领域有广泛的应用前景。然而，hESCs 的应用受到其对冷冻和解冻过程的敏感性的限制。因此，这项新兴科学需要新的、可靠的保存方法，以便在冷冻和解冻后长期储存大量具有多能性的功能性 hESCs。在这里，我们使用改良的细胞培养基质，为大量贴壁 hESC 集落的冷冻保存提供了一种高效的基于表面的玻璃化方法。与悬浮冷冻保存相比，该技术可显著提高解冻后的存活率，并允许快速、精确地处理和储存细胞，表明分化率较低。
