Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel.
Acta Biomater. 2012 Jan;8(1):209-17. doi: 10.1016/j.actbio.2011.08.022. Epub 2011 Aug 30.
Naturally derived materials are becoming widely used in the biomedical field. Soy protein has advantages over various types of natural proteins employed for biomedical applications due to its low price, non-animal origin and relatively long storage time and stability. In the current study soy protein isolate (SPI) was investigated as a matrix for wound dressing applications. The antibiotic drug gentamicin was incorporated into the matrix for local controlled release and, thus, protection against bacterial infection. Homogeneous yellowish films were cast from aqueous solutions. After cross-linking they combined high tensile strength and Young's modulus with the desired ductility. The plasticizer type, cross-linking agent and method of cross-linking were found to strongly affect the tensile properties of the SPI films. Selected SPI films were tested for relevant physical properties and the gentamicin release profile. The cross-linking method affected the degree of water uptake and the weight loss profile. The water vapor transmission rate of the films was in the desired range for wound dressings (∼2300 g m(-2) day(-1)) and was not affected by the cross-linking method. The gentamicin release profile exhibited a moderate burst effect followed by a decreasing release rate which was maintained for at least 4 weeks. Diffusion was the dominant release mechanism of gentamicin from cross-linked SPI films. Appropriate selection of the process parameters yielded SPI wound dressings with the desired mechanical and physical properties and drug release behavior to protect against bacterial infection. These unique structures are thus potentially useful as burn and ulcer dressings.
天然衍生材料在生物医学领域的应用越来越广泛。由于其价格低廉、非动物来源以及相对较长的储存时间和稳定性，大豆蛋白在用于生物医学应用的各种天然蛋白中具有优势。在本研究中，大豆分离蛋白（SPI）被用作伤口敷料应用的基质。将抗生素药物庆大霉素掺入基质中，实现局部控制释放，从而防止细菌感染。将其从水溶液中浇铸成均匀的黄色薄膜。交联后，它们结合了高拉伸强度和杨氏模量以及所需的延展性。增塑剂类型、交联剂和交联方法被发现强烈影响 SPI 薄膜的拉伸性能。对选定的 SPI 薄膜进行了相关物理性能和庆大霉素释放曲线的测试。交联方法影响水吸收程度和失重曲线。薄膜的水蒸气透过率在伤口敷料的理想范围内（约 2300 g m(-2) day(-1))，不受交联方法的影响。庆大霉素的释放曲线表现出适度的突释效应，随后释放速率逐渐降低，至少维持 4 周。扩散是交联 SPI 薄膜中庆大霉素释放的主要机制。适当选择工艺参数可得到具有所需机械和物理性能以及药物释放行为的 SPI 伤口敷料，以防止细菌感染。这些独特的结构因此可能有用作烧伤和溃疡敷料。
