Ningrum Dwi Ratna, Hanif Wildan, Mardhian Deby Fajar, Asri Lia A T W
Materials Science and Engineering Research Group, Faculty of Mechanical and Aerospace Engineering, Institute Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia.
Department of Dental Materials Science and Technology, Faculty of Dentistry, Universitas Padjadjaran, Sumedang 45363, Indonesia.
Polymers (Basel). 2023 Jan 16;15(2):468. doi: 10.3390/polym15020468.
Hydrogel-based wound dressings are often chosen for healing diabetic foot ulcers (DFU) in combination with herbal extracts. leaf (MOL) extract is a potent herb containing antimicrobial and anti-inflammatory bioactive substances. In this work, wound dressings based on polyvinyl alcohol (PVA), MOL extract, and graphene oxide (GO) were developed for DFU wound dressing. The PVA/MOL/GO hydrogel was synthesized using four cycles of a freeze-thaw process with varying concentrations of MOL extract. All hydrogels showed a water content of 83-88% and an equilibrium swelling ratio between 155-171%. After degradation in phosphate-buffered saline, the hydrogels showed a more open porous structure. We observed a degradation rate of 26-28%. Although the increase in MOL extract reduced the tensile strength of the hydrogel, the addition of GO increased the tensile strength. The PVA/MOL/GO hydrogel showed the highest antibacterial activity, with a reduction of 94% Gram-positive and 82% Gram-negative . Finally, all samples possessed appropriate cytocompatibility with cell viability reaching 83-135% in 3T3L1 mouse fibroblast cells. This result was verified by in vitro wound-healing analysis performed by scratch assay. This study presents the potency of combined PVA, MOL, and GO as a biocompatible DFU wound dressing.
基于水凝胶的伤口敷料通常与草药提取物结合用于治疗糖尿病足溃疡（DFU）。桑叶（MOL）提取物是一种含有抗菌和抗炎生物活性物质的强效草药。在这项工作中，开发了基于聚乙烯醇（PVA）、MOL提取物和氧化石墨烯（GO）的伤口敷料用于DFU伤口敷料。通过四个冻融循环，使用不同浓度的MOL提取物合成了PVA/MOL/GO水凝胶。所有水凝胶的含水量为83-88%，平衡溶胀率在155-171%之间。在磷酸盐缓冲盐水中降解后，水凝胶呈现出更开放的多孔结构。我们观察到降解率为26-28%。虽然MOL提取物的增加降低了水凝胶的拉伸强度，但GO的添加增加了拉伸强度。PVA/MOL/GO水凝胶表现出最高的抗菌活性，革兰氏阳性菌减少了94%，革兰氏阴性菌减少了82%。最后，所有样品都具有适当的细胞相容性，在3T3L1小鼠成纤维细胞中的细胞活力达到83-135%。通过划痕试验进行的体外伤口愈合分析验证了这一结果。本研究展示了PVA、MOL和GO组合作为生物相容性DFU伤口敷料的潜力。
