Gattefossé SAS, Saint-Priest, France.
Eur J Dermatol. 2011 Nov-Dec;21(6):851-63. doi: 10.1684/ejd.2011.1494.
Confocal Raman spectroscopy is a technique with considerable potential for the non-invasive study of biological tissues and skin samples in vitro or in vivo. It can be used to study skin physiology and possible pathological conditions and to obtain data about molecular composition and the structure of skin, for example, water content, moisturization and changes in the skin barrier function can all be observed. In-depth measurements also allow biopharmaceutical studies, such as analyzing the rate of penetration of a drug and the biochemical changes that may be induced by an applied formulation. Confocal Raman microspectroscopy is now at such a stage of refinement that it opens up new vistas. The big leap forward in its ease of use enables this technology to be used as an analytical method by more and more non-specialist laboratories. This review gives an overview of the state of the art of this technology by presenting an update on the principles of Raman spectroscopy and then by looking at examples of new developments in in vivo and in vitro applications.
共聚焦拉曼光谱是一种具有很大潜力的技术，可用于对生物组织和皮肤样本进行非侵入性的体外或体内研究。它可用于研究皮肤生理学和可能的病理状况，并获取有关皮肤分子组成和结构的数据，例如，可以观察到水分含量、保湿和皮肤屏障功能的变化。深入的测量还可以进行生物制药研究，例如分析药物的渗透速度以及应用配方可能引起的生化变化。共聚焦拉曼显微光谱技术现在已经达到了这样一个精细的阶段，为人们开辟了新的视野。它在易用性方面的巨大飞跃使这项技术能够被越来越多的非专业实验室用作分析方法。这篇综述通过介绍拉曼光谱的原理来概述该技术的最新进展，然后再通过观察体内和体外应用的新进展示例，对该技术进行了全面的介绍。
