Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Sipailou 2#, Nanjing 210096, PR China.
Environ Pollut. 2011 Dec;159(12):3468-73. doi: 10.1016/j.envpol.2011.08.024. Epub 2011 Sep 13.
Extensive production and application of γ-Fe(2)O(3) magnetic nanoparticles (MNPs) has increased their potential risk on environment and human health. This report illustrates a genetic impact of γ-Fe(2)O(3) magnetic nanoparticles (MNPs) on Escherichia coli (E. coli). After 3000-generation incubation with MNPs addition, obvious genomic variations were revealed by using repetitive extragenic palindromic PCR (rep-PCR) DNA fingerprint technique. The physicochemical interactions between MNPs and bacteria could be responsible for such genomic responses. It was revealed that Fe(3+) concentration increased in the medium. Transmission electronic microscopy (TEM) and flow cytometry (FCM) analysis consistently demonstrated the occurrences of adsorption and membranes-internalization of MNPs outside and inside cells. Both increased Fe(3+) ion and the uptake of MNPs facilitated Fe binding with proteins and DNA strands, resulting in enhancing the mutation frequency of E. coli. Our results would be of great help to assessing the potential impact of MNPs on human and environment.
γ-Fe(2)O(3) 磁性纳米颗粒（MNPs）的大量生产和应用增加了其对环境和人类健康的潜在风险。本报告说明了 γ-Fe(2)O(3) 磁性纳米颗粒（MNPs）对大肠杆菌（E. coli）的遗传影响。经过 3000 代的孵育和 MNPs 添加，使用重复外回文 PCR（rep-PCR）DNA 指纹技术揭示了明显的基因组变异。MNPs 和细菌之间的物理化学相互作用可能是导致这种基因组反应的原因。结果表明，培养基中的 Fe(3+)浓度增加。透射电子显微镜（TEM）和流式细胞术（FCM）分析一致表明 MNPs 在外层和内层细胞中的吸附和膜内化。增加的 Fe(3+)离子和 MNPs 的摄取促进了 Fe 与蛋白质和 DNA 链的结合，从而提高了大肠杆菌的突变频率。我们的结果将有助于评估 MNPs 对人类和环境的潜在影响。
