Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, 22110, Jordan,
Cytotechnology. 2015 May;67(3):427-35. doi: 10.1007/s10616-014-9701-8. Epub 2014 Mar 19.
Metal oxide nanoparticles have been suggested as good candidates for the development of antibacterial agents. Cerium oxide (CeO2) and iron oxide (Fe2O3) nanoparticles have been utilized in a number of biomedical applications. Here, the antibacterial activity of CeO2 and Fe2O3 nanoparticles were evaluated on a panel of gram positive and gram negative bacteria in both the planktonic and biofilm cultures. Additionally, the effect of combining CeO2 and Fe2O3 nanoparticles with the broad spectrum antibiotic ciprofloxacin on tested bacteria was investigated. Thus, minimum inhibitory concentrations (MICs) of CeO2 and Fe2O3 nanoparticles that are required to inhibit bacterial planktonic growth and bacterial biofilm, were evaluated, and were compared to the MICs of the broad spectrum antibiotic ciprofloxacin alone or in the presence of CeO2 and Fe2O3 nanoparticles. Results of this study show that both CeO2 and Fe2O3 nanoparticles fail to inhibit bacterial growth and biofilm biomass for all the bacterial strains tested. Moreover, adding CeO2 or Fe2O3 nanoparticles to the broad spectrum antibiotic ciprofloxacin almost abolished its antibacterial activity. Results of this study suggest that CeO2 and Fe2O3 nanoparticles are not good candidates as antibacterial agents, and they could interfere with the activity of important antibiotics.
金属氧化物纳米粒子被认为是开发抗菌剂的良好候选物。氧化铈（CeO2）和氧化铁（Fe2O3）纳米粒子已经在许多生物医学应用中得到了利用。在这里，评估了 CeO2 和 Fe2O3 纳米粒子在浮游和生物膜培养物中对革兰氏阳性和革兰氏阴性细菌的抗菌活性。此外，还研究了将 CeO2 和 Fe2O3 纳米粒子与广谱抗生素环丙沙星结合对测试细菌的影响。因此，评估了抑制细菌浮游生长和细菌生物膜所需的 CeO2 和 Fe2O3 纳米粒子的最小抑菌浓度（MIC），并将其与单独使用广谱抗生素环丙沙星或在存在 CeO2 和 Fe2O3 纳米粒子时的 MIC 进行了比较。这项研究的结果表明，CeO2 和 Fe2O3 纳米粒子都不能抑制所有测试菌株的细菌生长和生物膜生物量。此外，向广谱抗生素环丙沙星中添加 CeO2 或 Fe2O3 纳米粒子几乎会使其抗菌活性丧失。这项研究的结果表明，CeO2 和 Fe2O3 纳米粒子不是作为抗菌剂的良好候选物，并且它们可能会干扰重要抗生素的活性。
