Advanced Science Institute, RIKEN, Wako, Saitama 351-0198, Japan.
Chem Commun (Camb). 2011 Oct 28;47(40):11231-3. doi: 10.1039/c1cc14782j. Epub 2011 Sep 15.
Tungsten oxidation worked as a simple chemical reaction for the effective detection of 5-hydroxymethylcytosine in DNA, distinguishing it from its epigenetic precursors, 5-methylcytosine and unmethylated cytosine. The tungsten-oxidation product obtained from 5-hydroxymethylcytosine was trihydroxylated thymine and was detected as a cleavage band in gel electrophoresis after treatment with hot piperidine.
钨氧化反应可作为一种简单的化学反应，用于有效检测 DNA 中的 5-羟甲基胞嘧啶，将其与表观遗传前体 5-甲基胞嘧啶和未甲基化的胞嘧啶区分开来。从 5-羟甲基胞嘧啶中获得的钨氧化产物是三羟基化胸腺嘧啶，用哌啶热处理后在凝胶电泳中作为切割带被检测到。
