Key Laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China.
Chem Commun (Camb). 2020 Mar 12;56(21):3111-3114. doi: 10.1039/d0cc00167h.
The peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) is applied for the first time to the sensitive detection of locus-specific 5-hydroxymethylcytosine (5hmC) in DNA with low non-specific amplification. This strategy greatly shortens reaction time, improves sensitivity, and reduces non-specific amplification.
过氧钨酸盐氧化介导的两相扩增系统 (POM-TPAS) 首次应用于低非特异性扩增条件下灵敏检测 DNA 中的特异性 5-羟甲基胞嘧啶 (5hmC)。该策略大大缩短了反应时间，提高了灵敏度，降低了非特异性扩增。
