College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan, 430072, P. R. of China.
Org Biomol Chem. 2013 Jun 7;11(21):3568-72. doi: 10.1039/c3ob40447a. Epub 2013 Apr 30.
Treatment of DNA containing 5-formylcytosine with hot piperidine produced a cleaved band at the position of 5-formylcytosine in DNA sequences. After oxidation with KRuO4, 5-hydroxymethylcytosine could also be detected using the same method. Using our strategy, we could detect 5-hydroxymethylcytosine and 5-formylcytosine respectively.
用热哌啶处理含有 5-甲酰基胞嘧啶的 DNA，在 DNA 序列中 5-甲酰基胞嘧啶的位置产生一条断裂带。用 KRuO4 氧化后，也可以用同样的方法检测到 5-羟甲基胞嘧啶。使用我们的策略，我们可以分别检测到 5-羟甲基胞嘧啶和 5-甲酰基胞嘧啶。
