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基于过氧钨酸盐氧化和错配连接的环介导等温扩增技术对位点特异性5-羟甲基胞嘧啶进行快速且高度特异性的检测

Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP.

作者信息

Zhang Zhenhao, He Tong, Qi Yan, Dai Yuxuan, Lao Kejing, Gou Xingchun

机构信息

Shaanxi Key Laboratory of Brain Disorders, Shaanxi Provincial Key Laboratory of Infection and Immunity Diseases, Institute of Basic and Translational Medicine, Xi'an Medical University No. 1 Xin Wang Road Xi'an 710021 China

School of Chemistry and Chemical Engineering, Shaanxi Normal University Xi'an 710062 Shaanxi Province P. R. China.

出版信息

RSC Adv. 2022 Jul 7;12(31):19885-19889. doi: 10.1039/d2ra03310k. eCollection 2022 Jul 6.

DOI:10.1039/d2ra03310k
PMID:35865199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9261916/
Abstract

We have developed a rapid and specific method for site-specific 5-hydroxymethylcytosine (5hmC) quantification at single-base resolution. This bisulfite-free method integrates the peroxotungstate oxidization with the mismatched probe-assisted ligation to guarantee the specificity. Moreover, the high-efficiency LAMP also makes the proposed method suitable for the detection of low-content samples.

摘要

我们开发了一种快速且特异的方法，用于在单碱基分辨率下对位点特异性5-羟甲基胞嘧啶（5hmC）进行定量。这种无需亚硫酸氢盐的方法将过氧钨酸盐氧化与错配探针辅助连接相结合，以确保特异性。此外，高效的环介导等温扩增（LAMP）也使得该方法适用于低含量样本的检测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/a8615cdeb6ab/d2ra03310k-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/e2ae54f773ea/d2ra03310k-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/caba10c00ea6/d2ra03310k-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/deadad261b87/d2ra03310k-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/42e063a396d6/d2ra03310k-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/a8eb7e412d9d/d2ra03310k-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/a8615cdeb6ab/d2ra03310k-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/e2ae54f773ea/d2ra03310k-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/caba10c00ea6/d2ra03310k-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/deadad261b87/d2ra03310k-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/42e063a396d6/d2ra03310k-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/a8eb7e412d9d/d2ra03310k-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ff4/9261916/a8615cdeb6ab/d2ra03310k-f6.jpg

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Small Methods. 2021 May;5(5):e2100061. doi: 10.1002/smtd.202100061. Epub 2021 Apr 15.
3
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4
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