Department of Obstetrics & Gynecology, University of Michigan, Ann Arbor, MI 48109, USA.
J Infect Dis. 2011 Nov;204(9):1305-12. doi: 10.1093/infdis/jir541. Epub 2011 Sep 15.
The baboon (Papio hamadryas anubis) can be transcervically instrumented, facilitating studies of intrauterine contraception and reproductive tract infection. We sought to determine if the baboon could become infected with a single cervical inoculation of Chlamydia trachomatis.
Ten female baboons were randomized and inoculated cervically with C. trachomatis serovar E (or buffer alone). Animals underwent weekly clinical and laparoscopic evaluations for four weeks and at post-inoculation week 8, to monitor upper tract infection. Cervical culture and nucleic acid amplification testing (NAAT) were completed weekly throughout the study. Animals were euthanized at week 16 and the reproductive tracts were examined histologically.
All inoculated animals developed cervical infection. The average duration of positive NAAT results was 6.8 weeks (range 2-16). Two of eight (25%) animals tested positive from fallopian tube samples. Infected animals showed histological findings consistent with chlamydial infection, such as germinal centers. Five of ten animals seroconverted to C. trachomatis.
Baboons cervically inoculated once with C. trachomatis develop infection similar to humans, with a low incidence of upper tract infection. This novel model of Chlamydia infection closely resembles human disease and opens new avenues for studying the pathogenesis of sexually transmitted infections and contraceptive safety.
狒狒（Papio hamadryas anubis）可以经颈内器械操作，这有利于研究宫内避孕和生殖道感染。我们试图确定狒狒是否可以通过单次宫颈接种沙眼衣原体（Chlamydia trachomatis）而感染。
10 只雌性狒狒随机接受沙眼衣原体血清型 E（或缓冲液）的宫颈接种。动物在四周内每周接受临床和腹腔镜评估，并在接种后第 8 周进行上生殖道感染监测。每周完成宫颈培养和核酸扩增检测（NAAT）。动物在第 16 周被安乐死，对生殖道进行组织学检查。
所有接种动物均出现宫颈感染。NAAT 阳性结果的平均持续时间为 6.8 周（范围 2-16 周）。8 只动物中有 2 只（25%）从输卵管样本中呈阳性。感染动物表现出与衣原体感染一致的组织学发现，如生发中心。10 只动物中有 5 只血清转化为沙眼衣原体。
经颈内单次接种沙眼衣原体的狒狒会出现类似于人类的感染，上生殖道感染的发生率较低。这种新型衣原体感染模型与人类疾病非常相似，为研究性传播感染和避孕安全性的发病机制开辟了新途径。
