Fetissov Sergueï O
Digestive System and Nutrition Laboratory (ADEN EA4311), Rouen University, Rouen, France.
Methods Mol Biol. 2011;789:295-302. doi: 10.1007/978-1-61779-310-3_19.
This protocol describes an ELISA-based method for assaying serum levels of autoantibodies reactive with neuropeptides. The method allows for measuring relative amounts of free and bound, i.e., those present in immune complexes, autoantibodies using two types of sample buffers providing normal and dissociative conditions, respectively. This method can be applied to measure autoantibody levels directed against other than neuropeptide molecules and in a variety of biological fluids.
本方案描述了一种基于酶联免疫吸附测定（ELISA）的方法，用于检测与神经肽反应的自身抗体的血清水平。该方法可以分别使用提供正常条件和解离条件的两种类型的样品缓冲液，来测量游离和结合的自身抗体（即免疫复合物中存在的自身抗体）的相对量。该方法可用于测量针对神经肽分子以外的其他分子的自身抗体水平，并且适用于多种生物体液。
