20nm 高纳米狭缝中的电泳 DNA 传输：通过壁吸附运动的证据。

Electrokinetic DNA transport in 20 nm-high nanoslits: evidence for movement through a wall-adsorbed.

机构信息

Nanobioengineering Group, Institute for Bioengineering of Catalonia, Josep Samitier, Barcelona, Spain.

出版信息

Electrophoresis. 2011 Sep;32(18):2402-9. doi: 10.1002/elps.201100278. Epub 2011 Aug 26.

DOI:10.1002/elps.201100278

PMID:21922490

Abstract

The electrokinetic transport behavior of λ-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

摘要

本文研究了在存在短链 PVP 的情况下，λ-DNA（48 kbp）在 20nm 高的熔融二氧化硅纳米缝中的电动输运行为。迁移率和视频数据显示了许多在 DNA 通过凝胶或聚合物溶液传输时典型的现象，这表明在 DNA 传输途径中存在刚性迁移障碍。计算表明，几纳米厚的壁吸附 PVP（“纳米凝胶”）可以为 DNA 提供这样的刚性障碍基质。这种超薄的壁吸附聚合物层代表了一种用于电动 DNA 分离的新型基质。