二维 HSQC0 光谱法对海绵来源的Verrucosispora sp. 提取物中硫珊瑚素的选择性定量分析
Selective quantification by 2D HSQC0 spectroscopy of thiocoraline in an extract from a sponge-derived Verrucosispora sp.
机构信息
National Magnetic Resonance Facility at Madison, Biochemistry Department, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
出版信息
J Nat Prod. 2011 Oct 28;74(10):2295-8. doi: 10.1021/np200503c. Epub 2011 Sep 16.
Abstract
We recently developed a 2D 1H-13C HSQC0 approach to quantify individual chemicals in complex mixtures. The HSQC0 approach has been implemented in phase-cycled and gradient-selective versions. As in quantitative 1D NMR, the normalized integrated signal intensities in HSQC0 are proportional to the concentrations of individual chemicals in the mixture. We applied the HSQC0 approach to selectively quantify thiocoraline present at a level of 1% w/w in an extract from a Verrucosispora sp. isolated from the sponge Chondrilla caribensis f. caribensis. We expect that this approach can be used to quantify other natural products of interest in extracts without prior purification.
摘要
我们最近开发了一种 2D 1H-13C HSQC0 方法，用于定量分析复杂混合物中的单个化学物质。HSQC0 方法已经实现了相循环和梯度选择版本。与定量 1D NMR 一样，HSQC0 中归一化积分信号强度与混合物中各个化学物质的浓度成正比。我们应用 HSQC0 方法选择性地定量分析了从 Caribensis 海绵中分离出的 Verrucosispora sp. 提取物中含量为 1% w/w 的硫珊瑚素。我们预计，该方法可用于在不进行预先纯化的情况下，对提取物中其他感兴趣的天然产物进行定量分析。
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