Chang C, Martin R G, Livingston D M, Luborsky S W, Hu C P, Mora P T
J Virol. 1979 Jan;29(1):69-75. doi: 10.1128/JVI.29.1.69-75.1979.
The simian virus 40 (sv40) tumor antigen (T-antigen) and tumor-specific transplantation antigen (TSTA) have been partially purified and studied to clarify their relationship. The T-antigen and the TSTA were partially purified from nuclei of SV AL/N cells, and SV40-transformed mouse embryo fibroblast line, by precipitation with ammonium sulfate and chromatography on DEAE- and DNA-cellulose. The T-antigen was assayed by complement fixation, and the TSTA was assayed by its ability to immunize mice against SV40-containing ascites tumor cells. When T-antigen- and TSTA-containing preparations were sedimented through sucrose gradients, each antigen had a major peak of activity at a sedimentation coefficient of 6.7 and minor peaks in other regions. Antiserum against T-antigen (from tumor-bearing hamsters) immunoprecipitated the TSTA activity. A preparation of T-antigen from human SV80 cells, which exhibited only one protein band after sodium dodecylsulfate-polyacrylamide gel electrophoresis, had TSTA activity when as little as 0.6 microgram of protein per mouse was used for immunization. These experiments demonstrate that the T-antigen, the product of the SV40 early A gene is capable of inducing specific immunity against transplantation of SV40-transformed tumor cells in mice.
为阐明猿猴病毒40（SV40）肿瘤抗原（T抗原）与肿瘤特异性移植抗原（TSTA）之间的关系，对它们进行了部分纯化和研究。通过硫酸铵沉淀以及在DEAE - 纤维素和DNA - 纤维素上的层析，从SV AL/N细胞（一种SV40转化的小鼠胚胎成纤维细胞系）的细胞核中部分纯化了T抗原和TSTA。通过补体结合试验检测T抗原，通过其免疫小鼠抵抗含SV40腹水肿瘤细胞的能力检测TSTA。当含T抗原和TSTA的制剂通过蔗糖梯度沉降时，每种抗原在沉降系数为6.7处有一个主要活性峰，在其他区域有较小的峰。抗T抗原的血清（来自荷瘤仓鼠）免疫沉淀了TSTA活性。来自人SV80细胞的T抗原制剂，在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳后仅显示一条蛋白带，当每只小鼠仅用0.6微克蛋白质进行免疫时就具有TSTA活性。这些实验表明，SV40早期A基因的产物T抗原能够在小鼠中诱导针对SV40转化肿瘤细胞移植的特异性免疫。
