Reddy V B, Tevethia S S, Tevethia M J, Weissman S M
Proc Natl Acad Sci U S A. 1982 Mar;79(6):2064-7. doi: 10.1073/pnas.79.6.2064.
To understand the role of various functional domains of simian virus 40 early tumor antigens, we have cloned and introduced into mouse cells portions of early simian virus 40 DNA. Two types of truncated large tumor antigen (33 and 12.3 kilodaltons), as well as small tumor antigen, were identified by immunoprecipitation. Both truncated large tumor antigens have been found to be overproduced with respect to the small tumor antigen, although the 12.3-kilodalton truncated large tumor antigen was more stable than the 33-kilodalton one. Nonviral 53-kilodalton protein was not found associated with either truncated large tumor antigen in immunoprecipitations.
为了解猴病毒40早期肿瘤抗原各功能结构域的作用，我们克隆了猴病毒40早期DNA的部分片段并将其导入小鼠细胞。通过免疫沉淀鉴定出了两种截短型大肿瘤抗原（33千道尔顿和12.3千道尔顿）以及小肿瘤抗原。发现两种截短型大肿瘤抗原相对于小肿瘤抗原均过量产生，尽管12.3千道尔顿的截短型大肿瘤抗原比33千道尔顿的更稳定。在免疫沉淀中未发现非病毒53千道尔顿蛋白与任何一种截短型大肿瘤抗原相关联。
