Department of Biochemistry, University of Washington, Seattle, Washington, USA.
Nat Struct Mol Biol. 2011 Sep 18;18(10):1175-7. doi: 10.1038/nsmb.2119.
Following the failure of a wide range of attempts to solve the crystal structure of M-PMV retroviral protease by molecular replacement, we challenged players of the protein folding game Foldit to produce accurate models of the protein. Remarkably, Foldit players were able to generate models of sufficient quality for successful molecular replacement and subsequent structure determination. The refined structure provides new insights for the design of antiretroviral drugs.
在尝试通过分子置换法解决 M-PMV 逆转录病毒蛋白酶晶体结构的广泛努力失败后，我们向蛋白质折叠游戏 Foldit 的玩家发起挑战，要求他们生成该蛋白的精确模型。令人瞩目的是，Foldit 玩家成功生成了足以进行分子置换和后续结构测定的高质量模型。经修正的结构为设计抗逆转录病毒药物提供了新的见解。
