Veseli Enis, Staka Gloria, Tovani-Palone Marcos Roberto
Department of Prosthodontics, Dental School, Faculty of Medicine, University of Pristina, Pristina, Kosovo.
University Dentistry Clinical Center of Kosovo, Pristina, Kosovo.
BDJ Open. 2023 Feb 18;9(1):7. doi: 10.1038/s41405-023-00133-z.
This pilot study aimed to evaluate red-complex bacteria (RCB) loads in edentulous patients, before and after dentures' insertion.
Thirty patients were included in the study. Deoxyribonucleic acid (DNA) isolated from bacterial samples were obtained from the dorsum of the tongue before and 3 months after complete dentures (CDs) insertion in order to identify the presence of RCB (Tannerella forsythia, Porphyromonas gingivalis, and Treponema denticola) and quantify their loads, using real-time polymerase chain reaction (RT-PCR). Bacterial loads were represented as "Lg (genome equivalents/sample)" and the data classified according to the "ParodontoScreen" test.
Significant changes in bacterial loads were observed before and 3 months after the CDs insertion for: P. gingivalis (0.40 ± 0.90 vs 1.29 ± 1.64, p = 0.0007), T. forsythia (0.36 ±0.94 vs 0.87 ± 1.45, p = 0.005), and T. denticola (0.11 ± 0.41 vs 0.33 ± 0.75, p = 0.03). Before the CDs insertion, all patients had a normal bacterial prevalence range (100%) for all analyzed bacteria. Three months after the insertion, 2 (6.7%) of them had a moderate bacterial prevalence range for P. gingivalis, while 28 (93.3%) had a normal bacterial prevalence range.
The use of CDs has a significant impact on increasing RCB loads in edentulous patients.
本试点研究旨在评估无牙颌患者在佩戴假牙前后红色复合体细菌（RCB）的负荷情况。
本研究纳入了30名患者。从全口义齿（CDs）佩戴前及佩戴3个月后的舌背采集细菌样本，提取脱氧核糖核酸（DNA），以鉴定RCB（福赛坦氏菌、牙龈卟啉单胞菌和具核梭杆菌）的存在并使用实时聚合酶链反应（RT-PCR）对其负荷进行定量。细菌负荷以“Lg（基因组当量/样本）”表示，并根据“牙周筛查”测试对数据进行分类。
观察到CDs佩戴前及佩戴3个月后，以下细菌的负荷有显著变化：牙龈卟啉单胞菌（0.40±0.90 vs 1.29±1.64，p = 0.0007）、福赛坦氏菌（0.36±0.94 vs 0.87±1.45，p = 0.005）和具核梭杆菌（0.11±0.41 vs 0.33±0.75，p = 0.03）。在佩戴CDs前，所有患者所有分析细菌的患病率均在正常范围内（100%）。佩戴3个月后，其中2名患者（6.7%）牙龈卟啉单胞菌的患病率处于中等范围，而28名患者（93.3%）的患病率在正常范围内。
佩戴CDs对增加无牙颌患者的RCB负荷有显著影响。
