Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA.
Philos Trans R Soc Lond B Biol Sci. 2011 Oct 27;366(1580):2902-9. doi: 10.1098/rstb.2011.0139.
The smallest ribozyme that carries out a complex group transfer is the sequence GUGGC-3', acting to aminoacylate GCCU-3' (and host a manifold of further reactions) in the presence of substrate PheAMP. Here, I describe the enzymatic rate, the characterization of about 20 aminoacyl-RNA and peptidyl-RNA products and the pathways of these GUGGC/GCCU reactions. Finally, the topic is evolution, and the potential implications of these data for the advent of translation itself.
能够进行复杂基团转移的最小核酶是 GUGGC-3'序列，在苯丙氨酰腺苷酸（PheAMP）存在的情况下，它能够使 GCCU-3'氨酰化（并进一步催化多种反应）。在这里，我将描述该酶的反应速率、约 20 种氨酰-RNA 和肽酰-RNA 产物的特性以及这些 GUGGC/GCCU 反应的途径。最后，本文将探讨这些数据对翻译本身出现的潜在影响，以及该课题在进化方面的意义。
