核糖体上解码速度和准确性的进化优化。
Evolutionary optimization of speed and accuracy of decoding on the ribosome.
机构信息
Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry, 37077 Goettingen, Germany.
出版信息
Philos Trans R Soc Lond B Biol Sci. 2011 Oct 27;366(1580):2979-86. doi: 10.1098/rstb.2011.0138.
Abstract
Speed and accuracy of protein synthesis are fundamental parameters for the fitness of living cells, the quality control of translation, and the evolution of ribosomes. The ribosome developed complex mechanisms that allow for a uniform recognition and selection of any cognate aminoacyl-tRNA (aa-tRNA) and discrimination against any near-cognate aa-tRNA, regardless of the nature or position of the mismatch. This review describes the principles of the selection-kinetic partitioning and induced fit-and discusses the relationship between speed and accuracy of decoding, with a focus on bacterial translation. The translational machinery apparently has evolved towards high speed of translation at the cost of fidelity.
摘要
蛋白质合成的速度和准确性是细胞生存能力的基本参数，也是翻译质量控制和核糖体进化的关键。核糖体发展出了复杂的机制，使得任何同功的氨酰-tRNA（aa-tRNA）都能被均匀识别和选择，而任何近同功的 aa-tRNA 都会被区分，无论错配的性质或位置如何。本文综述了选择-动力学分配和诱导契合的原理，并讨论了解码速度和准确性之间的关系，重点是细菌翻译。翻译机制显然已经进化到了以保真度为代价的高速翻译。
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