Centre for Ionics University of Malaya, Physics Department, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Int J Nanomedicine. 2011;6:1817-23. doi: 10.2147/IJN.S23392. Epub 2011 Aug 30.
Three-dimensional assembly of graphene hydrogel is rapidly attracting the interest of researchers because of its wide range of applications in energy storage, electronics, electrochemistry, and waste water treatment. Information on the use of graphene hydrogel for biological purposes is lacking, so we conducted a preliminary study to determine the suitability of graphene hydrogel as a substrate for cell growth, which could potentially be used as building blocks for biomolecules and tissue engineering applications.
A three-dimensional structure of graphene hydrogel was prepared via a simple hydrothermal method using two-dimensional large-area graphene oxide nanosheets as a precursor.
The concentration and lateral size of the graphene oxide nanosheets influenced the structure of the hydrogel. With larger-area graphene oxide nanosheets, the graphene hydrogel could be formed at a lower concentration. X-ray diffraction patterns revealed that the oxide functional groups on the graphene oxide nanosheets were reduced after hydrothermal treatment. The three-dimensional graphene hydrogel matrix was used as a scaffold for proliferation of a MG63 cell line.
Guided filopodia protrusions of MG63 on the hydrogel were observed on the third day of cell culture, demonstrating compatibility of the graphene hydrogel structure for bioapplications.
由于在储能、电子、电化学和废水处理等领域的广泛应用，石墨烯水凝胶的三维组装迅速引起了研究人员的兴趣。关于石墨烯水凝胶在生物方面应用的信息还很缺乏，因此我们进行了一项初步研究，以确定石墨烯水凝胶作为细胞生长基底的适用性，这可能被用作生物分子和组织工程应用的构建块。
通过简单的水热法，以二维大面积氧化石墨烯纳米片为前体制备了三维石墨烯水凝胶结构。
氧化石墨烯纳米片的浓度和横向尺寸影响水凝胶的结构。使用较大面积的氧化石墨烯纳米片，可以在较低的浓度下形成石墨烯水凝胶。X 射线衍射图谱表明，水热处理后氧化石墨烯纳米片上的氧化物官能团被还原。三维石墨烯水凝胶基质被用作 MG63 细胞系增殖的支架。
在细胞培养的第三天观察到 MG63 在水凝胶上引导丝状伪足的突起，表明石墨烯水凝胶结构适用于生物应用。
