Department of Chemistry, Stanford University, Stanford, California 94305, USA.
J Am Chem Soc. 2011 May 4;133(17):6825-31. doi: 10.1021/ja2010175. Epub 2011 Apr 8.
We developed nanosized, reduced graphene oxide (nano-rGO) sheets with high near-infrared (NIR) light absorbance and biocompatibility for potential photothermal therapy. The single-layered nano-rGO sheets were ∼20 nm in average lateral dimension, functionalized noncovalently by amphiphilic PEGylated polymer chains to render stability in biological solutions and exhibited 6-fold higher NIR absorption than nonreduced, covalently PEGylated nano-GO. Attaching a targeting peptide bearing the Arg-Gly-Asp (RGD) motif to nano-rGO afforded selective cellular uptake in U87MG cancer cells and highly effective photoablation of cells in vitro. In the absence of any NIR irradiation, nano-rGO exhibited little toxicity in vitro at concentrations well above the doses needed for photothermal heating. This work established nano-rGO as a novel photothermal agent due to its small size, high photothermal efficiency, and low cost as compared to other NIR photothermal agents including gold nanomaterials and carbon nanotubes.
我们开发了具有高光热转换效率和生物相容性的纳米尺寸还原氧化石墨烯（nano-rGO）薄片，用于潜在的光热治疗。单层的 nano-rGO 薄片的平均横向尺寸约为 20nm，通过两亲性的 PEG 化聚合物链非共价功能化，以在生物溶液中赋予稳定性，并表现出比非还原的、共价 PEG 化的 nano-GO 高 6 倍的近红外（NIR）吸收。将带有 Arg-Gly-Asp（RGD）基序的靶向肽连接到 nano-rGO 上，可使 U87MG 癌细胞选择性摄取，并在体外高效光消融细胞。在没有任何近红外照射的情况下，nano-rGO 在体外的浓度远高于光热加热所需的剂量时，表现出很小的毒性。与其他近红外光热剂（包括金纳米材料和碳纳米管）相比，nano-rGO 由于其尺寸小、光热效率高、成本低，因此被确立为一种新型的光热剂。
